Identification and advantage of metabotropic glutamate receptor 5 antibody
Listening to the steps of winter, the leaves are red and the thoughts are flowing; the water is sending me a message, happiness is waiting for you; the frost is cold and sloppy, and the good feelings are gathered; when the greetings are lightly heard, the sound is blessed. Li Dong gives you all the best, I wish you all the best! Next, introduce the identification and advantages of metabotropic glutamate receptor 5 antibody.
Identification of antibodies:
1) Antibody titer identification: There are many methods for identifying titer, including tube agglutination reaction, agar diffusion test, enzyme-linked immunosorbent assay, and the like. Antibodies prepared by commonly used antigens generally have a method for identifying titers for comparison. For example, the titer of the anti-antibody is generally determined by an agar diffusion test.
2) Specific identification of antibodies: The specificity of an antibody refers to the ability to recognize with a corresponding antigen or an approximate antigenic substance. The specificity of the antibody is high and its recognition ability is strong. The specificity of measurement is usually expressed as a cross-reaction rate. The cross-reaction rate can be determined by a competition inhibition test. The competitive inhibition curves were prepared with different concentrations of antigen and approximate antigen, and the respective binding rates were calculated, and the respective concentrations at IC50 were determined, and the cross-reaction rate was calculated according to the formula.
3) Affinity of the eukaryotic translation initiation factor 2C2 antibody: refers to the degree of binding of the antibody to the antigen. The level of affinity is determined by the size of the antigen molecule, the suitability of the steric configuration between the binding site of the antibody molecule and the antigenic determinant. The intermolecular forces that help maintain the stability of the antigen-antibody complex are hydrogen bonds, hydrophobic bonds, Coulomb forces, van der Waals forces, and spatial repulsion of oppositely charged genes. Affinity is often expressed as the affinity constant K, and the unit of K is L/mol.
The following advantages:
1. In the aspect of antibody purification, using affinity chromatography technology, the technology can achieve purity of more than 99%, thereby ensuring the rigor of detection and the accuracy, sensitivity and specificity of the results;
2. The antibody is suitable for a variety of scientific research experiments;
The company's antibody range is complete, to meet the needs of different customers of different experiments.
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